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The joint laboratory CNRS / Bayer CropScience

The joint laboratory CNRS / Bayer CropScience

The joint laboratory CNRS / Bayer CropScience has been created at the instigation of both private and public partners to encourage the development of novel agronomic solutions by the reinforcement of the interactions and exchanges between fundamental and applied research. Since 15 years, the results that have been obtained led to the publication of more than 100 scientific papers and of several patents, thereby strengthening competitiveness of the company. Moreover the joint laboratory CNRS / Bayer CropScience has acquired an internationally recognized expertise in plant, seed, and fungus-physiology, biochemistry and molecular biology. This knowledge and expertise has been applied in the fields of herbicides, fungicides, seed treatments and agronomic and quality traits. Because of the status of this laboratory, research actions have always been planned in common, taking into account interest of both public and private partners. This success is due to a volunteer effort for developing pluri-disciplinary approaches involving researchers of different cultures, acting on common projects. The laboratory is located within CRLD (Centre de Recherche de la Dargoire, Lyon, France). It operates in close contact with this research center and also with the other BCS research centers.

Selected papers published by the “Joint laboratory CNRS / Bayer CropScience”


Holdsworth MJ, Finch-Savage WE, Grappin P, Job D (2008) Post-genomics dissection of seed dormancy and germination. Trends in Plant Science 13, 7-13.

Rajjou L, Lovigny Y, Groot SP, Belghazi M, Job C, Job D (2008) Proteome-wide characterization of seed aging in Arabidopsis. A comparison between artificial and natural aging protocols. Plant Physiology, 148: 620-641


Oracz K, El-Maarouf Bouteau H, Farrant JM, Cooper K, Belghazi M, Job C, Job D, Corbineau F, Bailly C (2007) ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation. The Plant Journal 50: 452-465.

Fudal I, Collemare J, Böhnert HU, Melayah D, Lebrun M-H (2007) Expression of M. grisea avirulence gene ACE1 is connected to the initiation of appressorium mediated penetration. Eukaryotic Cell 6, 546-54.

Rajjou L, Miché L, Huguet R, Job C, Job D (2007) The use of proteome and transcriptome profiling in the understand of seed germination and identification of intrinsic markers determining seed quality, germination efficiency and early seedling vigor. In Adkins SW, Ahmore S, Navie S (eds) Seeds. Biology. Development and Ecology, CAB International publishing, Cambridge, USA, pp 149-158

Rajjou L, Lovigny Y, Job C, Belghazi M, Groot SPC, Job D (2007) Seed quality and germination. In Adkins SW, Ahmore S, Navie S (eds) Seeds. Biology. Development and Ecology, CAB International publishing, Cambridge, USA, pp. 324-332.

Dufourmantel N, Dubald M, Matringe M, Canard H, Garcon F, Job C, Kay E, Wisniewski JP, Ferullo JM, Pelissier B, Sailland A, Tissot G (2007) Generation and characterization of soybean and marker-free tobacco plastid transformants over-expressing a bacterial 4-hydroxyphenylpyruvate dioxygenase which provides strong herbicide tolerance. Plant Biotechnology Journal 5, 118–133.


Rajjou L, Belghazi M, Huguet R, Robin C, Moreau A, Job C, Job D (2006) Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms. Plant Physiology 141, 910-923.

Chibani K, Ali-Rachedi S, Job C, Job D, Jullien M, Grappin P (2006) Proteomic analysis of seed dormancy in Arabidopsis. Plant Physiology 142, 1493-1510.

Veneault-Fourrey C, Lambou K, Lebrun MH (2006) Fungal Pls1 tetraspanins as key factors of penetration into host plants: a role in re-establishing polarized growth in the appressorium? FEMS Microbiol Lett 256, 179–184.

Vergne E, Ballini E, Marques S, Sidi Mammar B, Droc G, Gaillard S, Bourot S, DeRose R, Tharreau D, Nottéghem J-L, Lebrun M-H, Morel J-B (2006) Early and specific gene expression triggered by rice resistance gene Pi33 in response to infection by ACE1 avirulent blast fungus. New Phytologist 174, 159-71.

Rajjou L, Gallardo K, Job C, Job D (2006) Proteomics of seed development. In Finnie C (ed) Plant proteomics, Annual Plant Reviews 28. Blackwell publishing, Oxford, pp. 151-184.


Dean RA, Talbot NJ, Ebbole DJ, Farman ML, Mitchell T, Orbach M, Thon M, Kulkarni, R, Xu J-R, Pan H, Read NR, Lee Y-H, Carbone I, Brown D, Yee Y, Donofrio N, Soanes D, Djonovic S, Kolomiets E, Rehmeyer C, Li W, Harding M, Kim S, Lebrun MH, Bohnert H, Coughlin S, Butler J, Calvo S, Ma L-J, Nicol R, Purcell S, Nusbaum C, Galagan JE, Birren BW (2005) The genome sequence of the rice blast fungus Magnaporthe grisea. Nature 434, 980-986.

Fudal I, Bohnert HU, Tharreau D, Lebrun MH (2005) Transposition of MINE, a composite retrotransposon, in the avirulence gene ACE1 of the rice blast fungus Magnaporthe grisea. Fungal Genet Biol 42, 761-772.

Job C, Rajjou L, Lovigny Y, Belghazi M, Job D (2005) Patterns of Protein Oxidation in Arabidopsis Seeds and during Germination. Plant Physiol138, 790-802


Bohnert HU, Fudal I, Dioh W, Tharreau D, Notteghem JL, Lebrun MH (2004) A putative polyketide synthase/peptide synthetase from Magnaporthe grisea signals pathogen attack to resistant rice. Plant Cell 16, 2499-2513.

Droux M (2004) Sulfur assimilation and the role of sulfur in plant metabolism: a survey.
Photosynth Res. 79, 331-348

Rajjou L, Gallardo K, Debeaujon I, Vandekerckhove J, Job C, Job D (2004) The effect of alpha-amanitin on the Arabidopsis seed proteome highlights the distinct roles of stored and neosynthesized mRNAs during germination. Plant Physiol 134, 1598-1613


Picciocchi A, Douce R, Alban C (2003) The plant biotin synthase reaction. Identification and characterization of essential mitochondrial accessory protein components. J Biol Chem 278, 24966-24975


Denis L, Grossemy M, Douce R, Alban C (2002) Molecular characterization of a second copy of holocarboxylase synthetase gene (hcs2) in Arabidopsis thaliana. J Biol Chem 277, 10435-10444

Gakière B, Denis L, Droux M, Job D (2002) Overexpression of cystathionine gamma-synthase in Arabidopsis thaliana leads to increased levels of methionine and S-methylmethionine. Plant Physiol Biochem 40, 119-126

Gallardo K, Job C, Groot SPC, Puype M, Demol H, Vandekerckhove J, Job D (2002) Proteomics of Arabidopsis seed germination. A comparative study of wild type and gibberellin deficient seeds. Plant Physiol 129, 823-837

Gallardo K, Job C, Groot SPC, Puype M, Demol H, Vandekerckhove J, Job D (2002) Importance of methionine biosynthesis for Arabidopsis seed germination and seedling growth. Physiol Plant 116, 238-247

Gourgues M, Clergeot PH, Veneault C, Cots J, Sibuet S, Brunet-Simon A, Levis C, Langin T, Lebrun MH (2002) A new class of tetraspanins in fungi. Biochem Biophys Res Commun 297, 1197-204.

Tabe LM, Droux M (2002) Limits to sulfur accumulation in transgenic lupin seeds expressing a foreign sulfur-rich protein. Plant Physiol 128, 1137-1148


Clergeot PH, Latorse MP, Laurans F, Pépin R, Tharreau D, Notteghem JL, Lebrun MH (2001) PLS1, a gene encoding a tetraspanin-like protein is required for penetration of host leaves by the rice blast fungus Magnaporthe grisea. Proc Natl Acad Sci USA 98, 6963-6968

Dumas R, Biou V, Halgand F, Douce R, Duggleby R (2001) Enzymology, structure and dynamics of acetohydroxy acid isomeroreductase. Accounts of Chemical Research 34, 399-408

Gallardo K, Job C, Groot SPC, Puype M, Demol H, Vandekerckhove J, Job D (2001) Proteomic analysis of Arabidopsis seed germination and priming. Plant Physiol 126, 835-848

Job C, Laugel S, Duval M, Gallardo K, Job D (2001) Biochemical characterization of atypical biotinylation domains in plant proteins. Seed Science Research 11, 149-161


Alban C, Job D, Douce R (2000) Biotin metabolism in plants. Ann Rev Plant Physiol Plant Mol Biol 51, 17-47

Bourgne S, Job C, Job D (2000) Sugar beet seed priming: solubilization of the basic subunit of 11-S globulin in individual seeds. Seed Science Research 10, 153-161

Capron I, Corbineau F, Dacher F, Job C, Côme D, Job D (2000) Sugar beet seed priming: effects of priming conditions on germination, solubilization of 11-S globulin and accumulation of LEA proteins. Seed Science Research 10, 243-254

Gakière B, Ravanel S, Droux M, Douce R, Job D (2000) Mechanisms to account for maintenance of the soluble methionine pool in transgenic Arabidopsis plants expressing antisense cystathionine gamma-synthase cDNA. CR Acad Sci (Paris) Sciences de la Vie 323, 841-851

Garcia I, Job D, Matringe M (2000) Inhibition of p-hydroxyphenylpyruvate dioxygenase by the diketonitrile of isoxafutole: a case of half-site reactivity Biochemistry 39, 7501-7507


Droux M, Ruffet M-L, Douce R, Job D (1998) Interactions between serine acetyl transferase and O-acetylserine(thiol) lyase in higher plants: structural and kinetic properties of the free and bound enzymes. Eur J Biochem 255, 235 – 245

Ravanel S, Gakière B, Job D, Douce R (1998) The specific features of methionine biosynthesis and metabolism in plants. Proc Natl Acad Sci USA 95, 7805-7812

Curien G, Job D, Douce R, Dumas R (1998) Allosteric activation of Arabidopsis threonine synthase by S-adenosylmethionine. Biochemistry 37, 13212-13221

Halgand F, Vives F, Dumas R, Biou V, Andersen J, Andrieu J-P, Cantegril R, Gagnon J, Douce R, Forest E, Job D (1998) Kinetic and mass spectrometric analyses of the interactions between plant acetohydroxy acid isomeroreductase and thiadiazole derivatives. Biochemistry 37, 4773 – 4781


Biou V, Dumas R, Pebay-Peyroula E, Cohen-Addad C, Job D, Douce R (1997) Three-dimensional structure of acetohydroxyacid isomeroreductase from spinach chloroplasts at 2.2 Å resolution: Structural characterization of herbicide-conjugating plant acetohydroxyacid isomeroreductase. EMBO J 16, 3405-3415

Dehaye L, Duval M, Viguier D, Yaxley J, Job D (1997) Cloning of the pea gene encoding SBP65, a seed-specific biotinylated protein. Plant Mol Biol 35, 605-621

Dunford HB, Job D, Jones P (1997) Thermodynamic trajectory of enzyme evolution. The ultimate speed of enzyme reactions. J Phys Chem 101, 4349-4350

Job C, Kersulec M, Ravasio L, Chareyre C, Pépin R, Job D (1997) The solubilization of the basic subunit of sugar beet globulin during priming and early germination. Seed Science Research 7, 225-243

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